Introduction to Mammalian Genome Special Issue: Genome Editing
نویسندگان
چکیده
منابع مشابه
Genome Editing Using Mammalian Haploid Cells
Haploid cells are useful for studying gene functions because disruption of a single allele can cause loss-of-function phenotypes. Recent success in generating haploid embryonic stem cells (ESCs) in mice, rats, and monkeys provides a new platform for simple genetic manipulation of the mammalian genome. Use of haploid ESCs enhances the genome-editing potential of the CRISPR/Cas system. For exampl...
متن کاملEditing DNA Methylation in the Mammalian Genome
Mammalian DNA methylation is a critical epigenetic mechanism orchestrating gene expression networks in many biological processes. However, investigation of the functions of specific methylation events remains challenging. Here, we demonstrate that fusion of Tet1 or Dnmt3a with a catalytically inactive Cas9 (dCas9) enables targeted DNA methylation editing. Targeting of the dCas9-Tet1 or -Dnmt3a ...
متن کاملIntroduction to Special Issue
Although recent frameworks for test validation have emphasized utilization as a key component (e.g. Bachman & Palmer, 2010; Chapelle, 2012; Kane, 1992), what happens in the sometimes messy contexts where assessments are carried out is often not documented. This is perhaps because test developers are more focused on defining and operationalizing test constructs than on issues of implementation. ...
متن کاملRNA-guided genome editing of mammalian cells.
The microbial CRISPR-Cas adaptive immune system can be harnessed to facilitate genome editing in eukaryotic cells (Cong L et al., Science 339, 819-823, 2013; Mali P et al., Science 339, 823-826, 2013). Here we describe a protocol for the use of the RNA-guided Cas9 nuclease from the Streptococcus pyogenes type II CRISPR system to achieve specific, scalable, and cost-efficient genome editing in m...
متن کاملPreface: Introduction to Special Issue
Demands for flexibility, greater variety of products, and advanced production processes complicate the logistics and planning of manufacturing systems; this also increases the number of alternatives that should be formalized in a model. The evaluation of all the possible alternatives of the planning problem is often considered an NP-hard combinatorial problem. Practical problems often contain n...
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ژورنال
عنوان ژورنال: Mammalian Genome
سال: 2017
ISSN: 0938-8990,1432-1777
DOI: 10.1007/s00335-017-9708-5